Mouse Gene Set: SCHLINGEMANN_SKIN_CARCINOGENESIS_TPA_UP

For the Human gene set with the same name, see SCHLINGEMANN_SKIN_CARCINOGENESIS_TPA_UP

Standard name SCHLINGEMANN_SKIN_CARCINOGENESIS_TPA_UP
Systematic name MM649
Brief description Up-regulated in murine dorsal skin cells at 6 h after treatment with the phorbol ester carcinogen TPA [PubChem=4792].
Full description or abstract Malignant transformation of mouse skin by chemical carcinogens and tumour promoters, such as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), is a multistage process that leads to squamous cell carcinoma (SCC) formation. In an effort to identify tumour-associated genes, we studied the influence of short-term TPA-treatment on the gene expression profile of murine skin. A comprehensive microarray with some 5,000 murine gene specific cDNA fragments was established and hybridised with pooled RNA derived from control and TPA-treated dorsal skin samples. Of these genes, 54 were up- and 35 were down-regulated upon TPA application. Additionally, we performed suppression subtractive hybridisation (SSH) with respective RNA pools to generate and analyse a cDNA library enriched for TPA-inducible genes. Expression data of selected genes were confirmed by quantitative real-time PCR and Northern blot analysis. Comparison of microarray and SSH data revealed that 26% of up-regulated genes identified by expression profiling matched with those present in the SSH library. Besides numerous known genes, we identified a large set of unknown cDNAs that represent previously unrecognised TPA-regulated genes in murine skin with potential function in tumour promotion. Additionally, some TPA-induced genes, such as Sprr1A, Saa3, JunB, Il4ralpha, Gp38, RalGDS and Slpi exhibit high basal level in advanced stages of skin carcinogenesis, suggesting that at least a subgroup of the identified TPA-regulated genes may contribute to tumour progression and metastasis.
Collection M2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 12640676   Authors: Schlingemann J,Hess J,Wrobel G,Breitenbach U,Gebhardt C,Steinlein P,Kramer H,Fürstenberger G,Hahn M,Angel P,Lichter P
Exact source Table 3
Related gene sets (show 1 additional gene sets from the source publication)

(show 10 gene sets from the same authors)
External links
Filtered by similarity ?
Source species Mus musculus
Contributed by John Newman (University of Washington)
Source platform or
identifier namespace		 MOUSE_SEQ_ACCESSION
Dataset references  
Download gene set format: grp | gmt | xml | json | TSV metadata
Compute overlaps ? (show collections to investigate for overlap with this gene set)
Compendia expression profiles ? NG-CHM interactive heatmaps
(Please note that clustering takes a few seconds)
Mouse Transcriptomic BodyMap compendium

Legacy heatmaps (PNG)
Mouse Transcriptomic BodyMap compendium
Advanced query Further investigate these 42 genes
Show members (show 46 source identifiers mapped to 42 genes)
Version history 2022.1.Mm: First Introduced.

See MSigDB license terms here. Please note that certain gene sets have special access terms.