Mouse Gene Set: NABA_MATRISOME_ANGIOGENIC_PANCREATIC_ISLETS

Standard name

NABA_MATRISOME_ANGIOGENIC_PANCREATIC_ISLETS

Systematic name

MM17052

Brief description

Matrisome proteins detected in significantly higher abundance during angiogenic stage of insulinoma progression compared to normal islets and relative to hyperplastic stage of insulinoma progression (RIP-Tag2 model).

Full description or abstract

In this study, we aimed to characterize changes in the extracellular matrix (ECM) composition during insulinoma progression using a quantitative proteomics approach. To do so, we chose a mouse model of insulinoma, a subtype of pancreatic neuroendocrine tumors, characterized by the expression of SV40 large T antigen (Tag) in pancreatic beta-cells driven by the rat insulin promoter (RIP) (RIP1-Tag2 model). This model follows a well-defined timeline of tumor progression: hyperplastic islets appear by 4 weeks of age, angiogenic islets by 7-9 weeks, solid tumors at 10 weeks, and large and invasive adenomas by 12-13 weeks. Using quantitative proteomics based on isobaric peptide labeling (iTRAQ), we profiled the ECM proteomes or matrisomes of various stages: normal pancreatic islets, hyperplastic islets, angiogenic pancreatic islets, and insulinomas. We focused on ECM and ECM-associated proteins along with proteins found in only one of the two replicates but with at least two unique peptides. Applying a moderated F-test, we identified proteins detected in statistically significantly different abundance in tumor samples (hyperplastic, angiogenic islets or isulinomas) as compared to normal pancreatic islets. The gene set lists the matrisome proteins detected in significantly higher abundance during the angiogenic stage of insulinoma progression as compared to normal islets and earlier hyperplastic stage of insulinoma progression.

Collection

M2: Curated
CGP: Chemical and Genetic Perturbations

Source publication

Pubmed 28071719 Authors: Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

Exact source

Supplementary Table 1: srep40495-s2.xls, Figure 3B-G: 35 ECM and ECM-associated proteins detected in significantly different abundance during insulinoma progression.

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