Human Gene Set: STEGER_ADIPOGENESIS_DN

For the Mouse gene set with the same name, see STEGER_ADIPOGENESIS_DN

Standard name STEGER_ADIPOGENESIS_DN
Systematic name M2271
Brief description Genes down-regulated during adipogenesis of 3T3-L1 cells (fibroblast).
Full description or abstract The histone H3 lysine 79 methyltransferase DOT1L/KMT4 can promote an oncogenic pattern of gene expression through binding with several MLL fusion partners found in acute leukemia. However, the normal function of DOT1L in mammalian gene regulation is poorly understood. Here we report that DOT1L recruitment is ubiquitously coupled with active transcription in diverse mammalian cell types. DOT1L preferentially occupies the proximal transcribed region of active genes, correlating with enrichment of H3K79 di- and trimethylation. Furthermore, Dot1l mutant fibroblasts lacked H3K79 di- and trimethylation at all sites examined, indicating that DOT1L is the sole enzyme responsible for these marks. Importantly, we identified chromatin immunoprecipitation (ChIP) assay conditions necessary for reliable H3K79 methylation detection. ChIP-chip tiling arrays revealed that levels of all degrees of genic H3K79 methylation correlate with mRNA abundance and dynamically respond to changes in gene activity. Conversion of H3K79 monomethylation into di- and trimethylation correlated with the transition from low- to high-level gene transcription. We also observed enrichment of H3K79 monomethylation at intergenic regions occupied by DNA-binding transcriptional activators. Our findings highlight several similarities between the patterning of H3K4 methylation and that of H3K79 methylation in mammalian chromatin, suggesting a widespread mechanism for parallel or sequential recruitment of DOT1L and MLL to genes in their normal on state.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 18285465   Authors: Steger DJ,Lefterova MI,Ying L,Stonestrom AJ,Schupp M,Zhuo D,Vakoc AL,Kim JE,Chen J,Lazar MA,Blobel GA,Vakoc CR
Exact source Fig. 5: mRNA arrow(s) down
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Source species Mus musculus
Contributed by Arthur Liberzon (MSigDB Team)
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identifier namespace		 MOUSE_GENE_SYMBOL
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Version history 3.1: First introduced

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