Human Gene Set: PECE_MAMMARY_STEM_CELL_DN


Standard name PECE_MAMMARY_STEM_CELL_DN
Systematic name M2535
Brief description The '3/3 signature': genes consistently down-regulated in all three pools of normal mammary stem cells (defined by their ability to retain the dye PKH26).
Full description or abstract Pathways that govern stem cell (SC) function are often subverted in cancer. Here, we report the isolation to near purity of human normal mammary SCs (hNMSCs), from cultured mammospheres, on the basis of their ability to retain the lipophilic dye PKH26 as a consequence of their quiescent nature. PKH26-positive cells possess all the characteristics of hNMSCs. The transcriptional profile of PKH26-positive cells (hNMSC signature) was able to predict biological and molecular features of breast cancers. By using markers of the hNMSC signature, we prospectively isolated SCs from the normal gland and from breast tumors. Poorly differentiated (G3) cancers displayed higher content of prospectively isolated cancer SCs (CSCs) than did well-differentiated (G1) cancers. By comparing G3 and G1 tumors in xenotransplantation experiments, we directly demonstrated that G3s are enriched in CSCs. Our data support the notion that the heterogeneous phenotypical and molecular traits of human breast cancers are a function of their CSC content.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 20074520   Authors: Pece S,Tosoni D,Confalonieri S,Mazzarol G,Vecchi M,Ronzoni S,Bernard L,Viale G,Pelicci PG,Di Fiore PP
Exact source Table 1S: sheet=hNMSC-signature; D in all 3 pools
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Source species Homo sapiens
Contributed by Arthur Liberzon (MSigDB Team)
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identifier namespace		 AFFY_HG_U133
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Version history 5.0: Renamed from PECE_MAMMARY_STEM_CELL_UP

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