Genes bound by ASCL1 in prostate cancer cell lines, and up-regulated in ASCL1 positive patient-driven xenografts.
Full description or abstract
We performed ChIPseq analysis on ASCL1 positive neuroendocrine prostate cancer cell lines 42D-ENZR and two sets of NCI-H660 as well as in LNCaP cells overexpressing ASCL1. Several thousand ASCL1 binding sites were identified following analysis of each ChIPseq. A total of 2,360 genes annotated to ASCL1 were found common among the four samples. We identified ASCL1 binding at bona fide ASCL1 regulated genes such as INSM1, ID4, HES6, CHGA and DLL4. Integrated ASCL1 ChIPseq data with transcriptomics (RNAseq) data, to identify a consensus ASCL1 transcriptome, we utilized RNAseq from publicly available ASCL1 (+) vs ASCL1 (?) patient driven xenografts from Labreque et al, 2019. 160 ASCL1-bound genes identified in prostate cancer ChIPseq studies also exhibited significantly higher mRNA expression (log2FoldChange?>?2) in the ASCL1 (+) compared to ASCL1 (?) LuCaP PDXs. These genes represent targets of ASCL1, where expression is likely directly regulated by ASCL1 binding.
Collection
C2: Curated CGP: Chemical and Genetic Perturbations
