Human Gene Set: NOURUZI_NEPC_ASCL1_TARGETS


Standard name NOURUZI_NEPC_ASCL1_TARGETS
Systematic name M46420
Brief description Genes bound by ASCL1 in prostate cancer cell lines, and up-regulated in ASCL1 positive patient-driven xenografts.
Full description or abstract We performed ChIPseq analysis on ASCL1 positive neuroendocrine prostate cancer cell lines 42D-ENZR and two sets of NCI-H660 as well as in LNCaP cells overexpressing ASCL1. Several thousand ASCL1 binding sites were identified following analysis of each ChIPseq. A total of 2,360 genes annotated to ASCL1 were found common among the four samples. We identified ASCL1 binding at bona fide ASCL1 regulated genes such as INSM1, ID4, HES6, CHGA and DLL4. Integrated ASCL1 ChIPseq data with transcriptomics (RNAseq) data, to identify a consensus ASCL1 transcriptome, we utilized RNAseq from publicly available ASCL1 (+) vs ASCL1 (?) patient driven xenografts from Labreque et al, 2019. 160 ASCL1-bound genes identified in prostate cancer ChIPseq studies also exhibited significantly higher mRNA expression (log2FoldChange?>?2) in the ASCL1 (+) compared to ASCL1 (?) LuCaP PDXs. These genes represent targets of ASCL1, where expression is likely directly regulated by ASCL1 binding.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 35477723   Authors: Nouruzi S,Ganguli D,Tabrizian N,Kobelev M,Sivak O,Namekawa T,Thaper D,Baca SC,Freedman ML,Aguda A,Davies A,Zoubeidi A
Exact source ChIPseq from current publication PMID (35477723), ChIPseq from Baca et al, 2021 (PMID 34599169) and RNAseq from Labreque et al, 2019 (PMID 31361600)
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Source species Homo sapiens
Contributed by Amina Zoubeidi (University of British Columbia and Vancouver Prostate Centre)
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identifier namespace		 HUMAN_GENE_SYMBOL
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Version history 2023.1.Hs: First Introduced.

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