Human Gene Set: MEBARKI_HCC_PROGENITOR_WNT_DN_CTNNB1_INDEPENDENT
Standard name
MEBARKI_HCC_PROGENITOR_WNT_DN_CTNNB1_INDEPENDENT
Systematic name
M38987
Brief description
Transcriptome of human HepaRG hepatocellular carcinoma liver progenitors in responses to a WNT3A-enriched microenvironment and dissection of pathways dependent on _-catenin and/or blocked by the SFRP-like Wnt inhibitor FZD8_CRD.
Full description or abstract
Methods: Liver progenitor cells were incubated in a WNT-enriched microenvironment for 72hrs (200 ng/ml mouse recombinant purified Wnt3A from R&D Systems). Gene pathways dependent on downstream _-catenin were studied by _-catenin knockdown with specific siRNA. Gene pathways blocked by extracellular SFRP-like Wnt inhibitors were studied by co-incubating cells with recombinant purified FZD8_CRD (300 ng/ml, from R&D Systems). Independent culture experiments performed in triplicate include untreated cells or cells incubated with scrambled siRNA or with _-catenin-specific siRNA or with FZD8_CRD, alone or in combination with Wnt3A.
Collection
C2: Curated CGP: Chemical and Genetic Perturbations
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and Regents of the University of California, subject to the terms and conditions of the
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