Human Gene Set: MEBARKI_HCC_PROGENITOR_FZD8CRD_DN


Standard name MEBARKI_HCC_PROGENITOR_FZD8CRD_DN
Systematic name M38993
Brief description Transcriptome of human HepaRG hepatocellular carcinoma liver progenitors in responses to a WNT3A-enriched microenvironment and dissection of pathways dependent on _-catenin and/or blocked by the SFRP-like Wnt inhibitor FZD8_CRD.
Full description or abstract Methods: Liver progenitor cells were incubated in a WNT-enriched microenvironment for 72hrs (200 ng/ml mouse recombinant purified Wnt3A from R&D Systems). Gene pathways dependent on downstream _-catenin were studied by _-catenin knockdown with specific siRNA. Gene pathways blocked by extracellular SFRP-like Wnt inhibitors were studied by co-incubating cells with recombinant purified FZD8_CRD (300 ng/ml, from R&D Systems). Independent culture experiments performed in triplicate include untreated cells or cells incubated with scrambled siRNA or with _-catenin-specific siRNA or with FZD8_CRD, alone or in combination with Wnt3A. In the absence of Wnt in the microenvironnment, soluble FZD8_CRD may dimerize with other cell surface FZD_CRD domains.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 27191501   Authors: Mebarki S,Désert R,Sulpice L,Sicard M,Desille M,Canal F,Dubois-Pot Schneider H,Bergeat D,Turlin B,Bellaud P,Lavergne E,Le Guével R,Corlu A,Perret C,Coulouarn C,Clément B,Musso O
Exact source GSM1677868; GSM1677876; GSM1677884 in GSE68633
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Source species Homo sapiens
Contributed by Orlando Musso (INSERM (Institut National de la Santé et de la Recherche Médicale), France.)
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identifier namespace		 Human_AGILENT_Array
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