Human Gene Set: MA_MYELOID_DIFFERENTIATION_DN

For the Mouse gene set with the same name, see MA_MYELOID_DIFFERENTIATION_DN

Standard name MA_MYELOID_DIFFERENTIATION_DN
Systematic name M1433
Brief description Genes down-regulated during myeloid differentiation induced by tretinoin (ATRA) [PubChem=444795] and IL3 [GeneID=3652] in the EML cell line (myeloid progenitor).
Full description or abstract With the goal of creating a resource for in-depth study of myelopoiesis, we have executed a 2-pronged strategy to obtain a complementary DNA (cDNA) clone set enriched in hematopoietic genes. One aspect is a library subtraction to enrich for underrepresented transcripts present at early stages of hematopoiesis. For this, a hematopoietic cDNA library from primary murine bone marrow cells enriched for primitive progenitors was used as tester. The subtraction used 10 000 known genes and expressed sequence tags (ESTs) as driver. The 2304 randomly picked clones from the subtracted cDNA libraries represent 1255 distinct genes, of which 622 (50%) are named genes, 386 (30%) match uncharacterized ESTs, and 247 (20%) are novel. The second aspect of our strategy was to complement this subtracted library with genes known to be involved in myeloid cell differentiation and function. The resulting cDNAs were arrayed on polylysine-coated glass slides. The microarrays were used to analyze gene expression in primary and cultured murine bone marrow-derived progenitors. We found expression of various types of genes, including regulatory cytokines and their receptors, signal transduction genes, and transcription factors. To assess gene expression during myeloid differentiation, we examined patterns of change during induced differentiation of EML cells. Several hundred of the genes underwent fluctuations in expression level during myeloid cell differentiation. The complete database, accessible on the World Wide Web at http://yale130132115135.med.yale.edu/, allows for retrieval of information regarding these genes. Our microarray allows for genomewide expression analysis of myeloid stem cells, which will help in defining the regulatory mechanisms of stem cell differentiation.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 12130493   Authors: Ma X,Husain T,Peng H,Lin S,Mironenko O,Maun N,Johnson S,Tuck D,Berliner N,Krause DS,Perkins AS
Exact source Table 8
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Source species Mus musculus
Contributed by Kevin Vogelsang (MSigDB Team)
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identifier namespace		 MOUSE_GENE_SYMBOL
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Version history 3.1: First introduced

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