Human Gene Set: MANALO_HYPOXIA_DN


Standard name MANALO_HYPOXIA_DN
Systematic name M18562
Brief description Genes down-regulated in response to both hypoxia and overexpression of an active form of HIF1A [GeneID=3091].
Full description or abstract Hypoxia-inducible factor 1 (HIF-1) activates transcription of genes encoding angiogenic growth factors, which are secreted by hypoxic cells and stimulate endothelial cells, leading to angiogenesis. To determine whether HIF-1 also mediates cell-autonomous responses to hypoxia, we have compared gene expression profiles in arterial endothelial cells cultured under nonhypoxic versus hypoxic conditions and in nonhypoxic cells infected with adenovirus encoding beta-galactosidase versus a constitutively active form of HIF-1alpha (AdCA5). There were 245 gene probes that showed at least 1.5-fold increase in expression in response to hypoxia and in response to AdCA5; 325 gene probes showed at least 1.5-fold decrease in expression in response to hypoxia and in response to AdCA5. The largest category of genes down-regulated by both hypoxia and AdCA5 encoded proteins involved in cell growth/proliferation. Many genes up-regulated by both hypoxia and AdCA5 encoded cytokines/growth factors, receptors, and other signaling proteins. Transcription factors accounted for the largest group of HIF-1-regulated genes, indicating that HIF-1 controls a network of transcriptional responses to hypoxia in endothelial cells. Infection of endothelial cells with AdCA5 under nonhypoxic conditions was sufficient to induce increased basement membrane invasion and tube formation similar to the responses induced by hypoxia, indicating that HIF-1 mediates cell-autonomous activation of endothelial cells.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 15374877   Authors: Manalo DJ,Rowan A,Lavoie T,Natarajan L,Kelly BD,Ye SQ,Garcia JG,Semenza GL
Exact source Table 2S
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Source species Homo sapiens
Contributed by Kate Stafford (MSigDB Team)
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identifier namespace		 AFFY_HG_U133
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Version history 3.0: Renamed from MANALO_HYPOXIA_DN

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