Human Gene Set: HOEK_PBMC_INACTIVATED_INFLUENZA_ADULT_7DY_DN


Standard name HOEK_PBMC_INACTIVATED_INFLUENZA_ADULT_7DY_DN
Systematic name M41146
Brief description Genes down-regulated in peripheral blood mononuclear cell 7d vs 0d in adults after exposure to Inactivated influenza vaccine , time point 7D. Comment: Down-regulated DE RNA transcripts (down >= 1.5x) shared between both TIV-vaccinated donors
Full description or abstract Systems biology is an approach to comprehensively study complex interactions within a biological system. Most published systems vaccinology studies have utilized whole blood or peripheral blood mononuclear cells (PBMC) to monitor the immune response after vaccination. Because human blood is comprised of multiple hematopoietic cell types, the potential for masking responses of under-represented cell populations is increased when analyzing whole blood or PBMC. To investigate the contribution of individual cell types to the immune response after vaccination, we established a rapid and efficient method to purify human T and B cells, natural killer (NK) cells, myeloid dendritic cells (mDC), monocytes, and neutrophils from fresh venous blood. Purified cells were fractionated and processed in a single day. RNA-Seq and quantitative shotgun proteomics were performed to determine expression profiles for each cell type prior to and after inactivated seasonal influenza vaccination. Our results show that transcriptomic and proteomic profiles generated from purified immune cells differ significantly from PBMC. Differential expression analysis for each immune cell type also shows unique transcriptomic and proteomic expression profiles as well as changing biological networks at early time points after vaccination. This cell type-specific information provides a more comprehensive approach to monitor vaccine responses.
Collection C7: Immunologic Signature
      VAX: HIPC Vaccine Response
Source publication Pubmed 25706537   Authors: Hoek KL,Samir P,Howard LM,Niu X,Prasad N,Galassie A,Liu Q,Allos TM,Floyd KA,Guo Y,Shyr Y,Levy SE,Joyce S,Edwards KM,Link AJ
Exact source S7 Dataset
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External links https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338067/bin/pone.0118528.s007.xlsx
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Source species Homo sapiens
Contributed by HIPC SIGNATURES (NIAID/HIPC SIGNATURES)
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identifier namespace		 HUMAN_GENE_SYMBOL
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Version history 7.3: First Introduced.

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