Human Gene Set: GSE44955_MCSF_VS_MCSF_AND_IL27_STIM_MACROPHAGE_DN


Standard name GSE44955_MCSF_VS_MCSF_AND_IL27_STIM_MACROPHAGE_DN
Systematic name M9701
Brief description Genes down-regulated in macrophage differentiated by: CSF1 [GeneID=1435] versus CSF1 [GeneID=1435] and IL27 [GeneID=246778].
Full description or abstract In this study, we hypothesized that IL-27 could induce the expression of novel miRNAs in macrophages which may have functional relevance in terms of anti-viral activity. In this study, primary monocytes were differentiated into macrophages using M-CSF (M-Mac) or with a combination of M-CSF and IL-27 (I-Mac) for seven days. Following this, total RNA was extracted from these cells and deep sequencing was performed, in parallel with gene expression microarrays. Using the novel miRNA discovery software, miRDeep, seven novel miRNAs were discovered in the macrophages, four of which were expressed higher in I-Mac (miRNAs 2.1, 8.1, 9.1 and 14.2) whilst three were detected in both M-Mac and I-Mac (miRNAs 9.3, 13.6 and 15.8). The expression of six of the seven novel miRNAs was highly correlated with qRT-PCR using specific primer/probes designed for the novel miRNAs. Gene expression microarray further demonstrated that a number of genes were potentially targeted by these differentially expressed novel miRNAs.
Collection C7: Immunologic Signature
      IMMUNESIGDB: ImmuneSigDB
Source publication Pubmed 23535375   Authors: Swaminathan S,Hu X,Zheng X,Kriga Y,Shetty J,Zhao Y,Stephens R,Tran B,Baseler MW,Yang J,Lempicki RA,Huang D,Lane HC,Imamichi T
Exact source GSE44955_3901_200_DN
Related gene sets (show 1 additional gene sets from the source publication)

(show 113 gene sets from the same authors)
External links
Filtered by similarity ?
Source species Homo sapiens
Contributed by Jernej Godec (Dana-Farber Cancer Institute)
Source platform or
identifier namespace		 HUMAN_GENE_SYMBOL
Dataset references (show 1 datasets)
Download gene set format: grp | gmt | xml | json | TSV metadata
Compute overlaps ? (show collections to investigate for overlap with this gene set)
Compendia expression profiles ? NG-CHM interactive heatmaps
(Please note that clustering takes a few seconds)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)

Legacy heatmaps (PNG)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
Advanced query Further investigate these 199 genes
Gene families ? Categorize these 199 genes by gene family
Show members (show 200 source identifiers mapped to 199 genes)
Version history 7.3: Moved to ImmuneSigDB sub-collection.

See MSigDB license terms here. Please note that certain gene sets have special access terms.