Human Gene Set: GSE21546_WT_VS_SAP1A_KO_DP_THYMOCYTES_DN
Standard name
GSE21546_WT_VS_SAP1A_KO_DP_THYMOCYTES_DN
Systematic name
M7544
Brief description
Genes down-regulated in untreated double positive thymocytes: wildtype versus ELK4 [GeneID=2005] knockout.
Full description or abstract
Removal of the transcription factor SAP1a member of the Ternary Complex Factor (TCF) group of transcription factors which in conjunction with Serum Response Factor (SRF) has been shown to have a profound effect on positive selection in the thymus. When another TCF Elk1 is knocked out in mice there is no effect on positive selection unless it is on a Sap1a KO background where the phenotype is very severe. We have stimulated isolated double positive T cells (DPs) with anti-CD3 to mimic positive selection and compared basal and stimulated transcription across the four genotypes to discover the downstream targets of Sap1a involved in positive selection.
