Human Gene Set: GREENBAUM_E2A_TARGETS_DN

For the Mouse gene set with the same name, see GREENBAUM_E2A_TARGETS_DN

Standard name GREENBAUM_E2A_TARGETS_DN
Systematic name M1457
Brief description Genes down-regulated in pre-B lymphocytes upon Cre-Lox knockout of E2A [GeneID=6929].
Full description or abstract The transcription factors encoded by the E2A gene have been shown to play essential roles in the initiation and progression of lymphocyte development. However, there is still a lack of comprehensive understanding of E2A downstream genes in B-cell development. We previously developed a gene tagging-based chromatin immunoprecipitation (ChIP) system to directly evaluate E2A target genes in B-cell development. Here, we have improved this ChIP strategy and used it in conjunction with microarray analysis on E2A-deficient pre-B-cell lines to determine E2A target genes in lymphocyte development. Both microarray data and ChIP studies confirmed that E2A directly controls IgH gene expression. The microarray assay also revealed genes that were significantly up-regulated after E2A disruption. ChIP analysis showed that E2A was most likely to be directly involved in repression of some of these target genes such as Nfil3 and FGFR2. An inducible E2A reconstitution system further demonstrated that E2A-mediated repression of Nfil3 and FGFR2 was reversible. Collectively, these findings indicate that E2A is a positive regulator for one set of genes and a negative regulator for another set of genes in developing B lymphocytes.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 15310760   Authors: Greenbaum S,Lazorchak AS,Zhuang Y
Exact source Table 1
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Source species Mus musculus
Contributed by Kevin Vogelsang (MSigDB Team)
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identifier namespace		 MOUSE_SEQ_ACCESSION
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Version history 3.1: First introduced


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