The process in which a relatively unspecialized cell acquires specialized features of a mesenchymal stem cell. A mesenchymal stem cell is a cell that retains the ability to divide and proliferate throughout life to provide progenitor cells that can differentiate into specialized mesenchymal cells. [CL:0002452, GOC:BHF]
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C5: Ontology GO: Gene Ontology GO:BP: GO Biological Process