Standard name |
LEE_METASTASIS_AND_RNA_PROCESSING_UP |
Systematic name |
M8887 |
Brief description |
Components of RNA post-transcriptional modification machinery up-regulated in MDA-MB-435 cells (breast cancer) whose metastatic potential has been reduced by expression of NME1 [GeneID=4830]. |
Full description or abstract |
The role of Gemin5 in alternative mRNA splicing, tumor cell motility, and proteomic instability was investigated. Isotope Capture Affinity Tag proteomic analysis was conducted on MDA-MB-435 tumor cells transfected with either a control vector (C-100) or the Nm23-H1 metastasis suppressor (H1-177). Ingenuity pathway analysis revealed that RNA posttranscriptional processing was the most prominent class of differentially expressed proteins. Within this category, overexpression of Acinus1, Poly(a) binding protein, HNRPA2B1, Bop1, and Gemin5 was confirmed in less metastatic H1-177 cells. Overexpression of the latter four proteins was also observed in the lower metastatic antisense Ezrin transfectant of a murine osteosarcoma model system, confirming the general relevance of the trends. Gemin5, a component of the spliceosomal complex, was chosen for further study. Analysis of global mRNA splicing by SpliceArray chips revealed that 16 genes were differentially spliced in C-100 compared with H1-177 cells; transient transfection of gemin5 into C-100 cells restored the splice pattern to that of H1-177 cells. Alternative splicing patterns for the engulfment and cell motility 1 and thrombospondin 4 genes were confirmed by semiquantitative reverse transcription-PCR. Gemin5 overexpression coordinately reduced C-100 cell motility by 50%, and siRNA-mediated reduction of Gemin5 expression increased the motility of H1-177 cells by 2-fold (P < 0.004). The data provide the first demonstration that alterations in the expression of a spliceosome protein can effect both specific splicing events and tumor cell motility. The data also show that changes in mRNA splicing patterns accompany metastatic progression, which may contribute to proteome instability. |
Collection |
C2: Curated CGP: Chemical and Genetic Perturbations |
Source publication |
Pubmed 18245461 Authors: Lee JH,Horak CE,Khanna C,Meng Z,Yu LR,Veenstra TD,Steeg PS |
Exact source |
Table 2S |
Related gene sets |
(show 2 additional gene sets from the source publication)
(show 12 gene sets from the same authors)
|
External links |
|
Filtered by similarity ?
|
|
Source species |
Homo sapiens |
Contributed by |
Jessica Robertson (MSigDB Team) |
Source platform or identifier namespace |
HUMAN_GENE_SYMBOL |
Dataset references |
|
Download gene set |
format: grp | gmt | xml | json | TSV metadata |
Compute overlaps ? |
(show collections to investigate for overlap with this gene set)
|
Compendia expression profiles ? |
NG-CHM interactive heatmaps
(Please note that clustering takes a few seconds)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
Legacy heatmaps (PNG)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
|
Advanced query |
Further investigate
these 16 genes
|
Gene families ? |
Categorize these
16 genes by gene family
|
Show members |
(show 17 source identifiers mapped to 16 genes)
Source Id |
NCBI (Entrez) Gene Id |
Gene Symbol |
Gene Description |
ACIN1 |
22985 |
ACIN1 |
apoptotic chromatin condensation induc... |
BOP1 |
23246 |
BOP1 |
BOP1 ribosomal biogenesis factor [Sour... |
COIL |
8161 |
COIL |
coilin [Source:HGNC Symbol;Acc:HGNC:2184] |
CSTF1 |
1477 |
CSTF1 |
cleavage stimulation factor subunit 1 ... |
DHX16 |
8449 |
DHX16 |
DEAH-box helicase 16 [Source:HGNC Symb... |
EXOSC8 |
11340 |
EXOSC8 |
exosome component 8 [Source:HGNC Symbo... |
FBL |
2091 |
FBL |
fibrillarin [Source:HGNC Symbol;Acc:HG... |
GEMIN5 |
25929 |
GEMIN5 |
gem nuclear organelle associated prote... |
GRSF1 |
2926 |
GRSF1 |
G-rich RNA sequence binding factor 1 [... |
HNRPA2B1 |
3181 |
HNRNPA2B1 |
heterogeneous nuclear ribonucleoprotei... |
NOP5/NOP58 |
|
|
|
PA2G4 |
5036 |
PA2G4 |
proliferation-associated 2G4 [Source:H... |
PABPC1 |
26986 |
PABPC1 |
poly(A) binding protein cytoplasmic 1 ... |
PPIG |
9360 |
PPIG |
peptidylprolyl isomerase G [Source:HGN... |
RAE1 |
8480 |
RAE1 |
ribonucleic acid export 1 [Source:HGNC... |
RNGTT |
8732 |
RNGTT |
RNA guanylyltransferase and 5'-phospha... |
SLBP |
7884 |
SLBP |
stem-loop histone mRNA binding protein... |
|
Version history |
3.0: First introduced
|